Terbium in PDB 6trt: Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C.

The structure of Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C., PDB code: 6trt was solved by P.Roversi, N.Zitzmann, R.Ibba, M.Hensen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	128.86 / 4.58
Space group	P 32 1 2
Cell size a, b, c (Å), α, β, γ (°)	148.799, 148.799, 235.546, 90.00, 90.00, 120.00
R / Rfree (%)	29.1 / 29.7

The binding sites of Terbium atom in the Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C. (pdb code 6trt). This binding sites where shown within 5.0 Angstroms radius around Terbium atom.
In total 3 binding sites of Terbium where determined in the Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C., PDB code: 6trt:
Jump to Terbium binding site number: 1; 2; 3;

Terbium binding site 1 out of 3 in the Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C.


Mono view


Stereo pair view

A full contact list of Terbium with other atoms in the Tb binding site number 1 of Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C. within 5.0Å range: probe atom residue distance (Å) B Occ A:Tb1601 b:0.9 occ:1.00 OD2 A:ASP1304 2.3 0.5 1.0 OD1 A:ASP1435 2.9 0.3 1.0 CG A:ASP1304 3.0 0.8 1.0 OD2 A:ASP1302 3.0 0.2 1.0 OD1 A:ASP1304 3.1 0.8 1.0 OD2 A:ASP1435 3.3 0.1 1.0 CG A:ASP1435 3.5 0.4 1.0 NZ A:LYS1446 3.8 0.5 1.0 CG A:ASP1302 3.8 0.2 1.0 O A:LEU1436 3.9 0.9 1.0 CB A:ASP1304 4.5 0.9 1.0 OD1 A:ASP1302 4.5 0.5 1.0 CE A:LYS1446 4.5 0.1 1.0 C A:LEU1436 4.6 0.8 1.0 CB A:ASP1302 4.6 0.1 1.0 N A:LEU1436 4.8 0.8 1.0 CB A:ASP1435 5.0 0.8 1.0

Terbium binding site 2 out of 3 in the Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C.


Mono view


Stereo pair view

A full contact list of Terbium with other atoms in the Tb binding site number 2 of Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C. within 5.0Å range: probe atom residue distance (Å) B Occ A:Tb1602 b:0.6 occ:1.00 OE2 A:GLU770 2.5 0.0 1.0 OE2 A:GLU774 3.1 76.3 1.0 OE1 A:GLU774 3.2 72.0 1.0 CD A:GLU774 3.2 73.4 1.0 CD A:GLU770 3.5 0.8 1.0 O A:GLU770 3.6 0.4 1.0 C A:GLU770 3.6 0.1 1.0 N A:THR771 3.7 0.2 1.0 CB A:GLU770 3.8 0.9 1.0 CA A:THR771 3.8 0.9 1.0 CG2 A:THR771 3.9 0.2 1.0 CG A:GLU770 4.0 0.4 1.0 CG A:GLU774 4.3 70.4 1.0 CA A:GLU770 4.4 0.6 1.0 CD1 A:LEU767 4.4 0.7 1.0 CB A:THR771 4.5 0.9 1.0 OE1 A:GLU770 4.5 0.5 1.0 CB A:GLU774 4.8 68.3 1.0

Terbium binding site 3 out of 3 in the Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C.


Mono view


Stereo pair view

A full contact list of Terbium with other atoms in the Tb binding site number 3 of Chaetomium Thermophilum Udp-Glucose Glucosyl Transferase (Uggt) Double Cysteine Mutant S180C/T742C. within 5.0Å range: probe atom residue distance (Å) B Occ A:Tb1603 b:83.1 occ:1.00 OE2 A:GLU716 2.6 0.4 1.0 OE1 A:GLU716 2.6 0.8 1.0 OE1 A:GLU713 2.8 0.9 1.0 CD A:GLU716 2.8 0.1 1.0 OD1 A:ASP818 3.4 0.6 1.0 OD2 A:ASP818 3.6 0.8 1.0 CG A:ASP818 3.6 1.0 1.0 CD A:GLU713 3.7 0.8 1.0 CB A:ALA817 3.9 0.9 1.0 N A:ASP818 4.0 0.7 1.0 OE2 A:GLU713 4.1 0.3 1.0 CG A:GLU716 4.1 0.9 1.0 N A:ALA817 4.3 1.0 1.0 CA A:ALA817 4.4 0.7 1.0 C A:ALA817 4.4 0.6 1.0 CB A:GLU716 4.5 97.9 1.0 CB A:ASP818 4.7 0.0 1.0 CA A:ASP818 4.8 0.7 1.0 CA A:GLU713 5.0 0.9 1.0

P.Roversi, N.Zitzmann. Clamping, Bending, and Twisting Inter-Domain Motions in the Misfold-Recognising Portion of Udp-Glucose:Glycoprotein Glucosyl-Transferase Biorxiv 2019.
DOI: 10.1101/2019.12.25.888438