Terbium in PDB 6zv9: Terbium(III)-Bound De Novo Tim Barrel-Ferredoxin Fold Fusion Dimer with 4-Glutamate Binding Site and Tryptophan Antenna (Tfd-Ee N6W)

The structure of Terbium(III)-Bound De Novo Tim Barrel-Ferredoxin Fold Fusion Dimer with 4-Glutamate Binding Site and Tryptophan Antenna (Tfd-Ee N6W), PDB code: 6zv9 was solved by S.Caldwell, I.Haydon, N.Piperidou, P.Huang, D.Hilvert, D.Baker, C.Zeymer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	43.970, 56.470, 71.280, 90.00, 101.69, 90.00
R / Rfree (%)	19.5 / 22.9

The binding sites of Terbium atom in the Terbium(III)-Bound De Novo Tim Barrel-Ferredoxin Fold Fusion Dimer with 4-Glutamate Binding Site and Tryptophan Antenna (Tfd-Ee N6W) (pdb code 6zv9). This binding sites where shown within 5.0 Angstroms radius around Terbium atom.
In total only one binding site of Terbium was determined in the Terbium(III)-Bound De Novo Tim Barrel-Ferredoxin Fold Fusion Dimer with 4-Glutamate Binding Site and Tryptophan Antenna (Tfd-Ee N6W), PDB code: 6zv9:

Terbium binding site 1 out of 1 in the Terbium(III)-Bound De Novo Tim Barrel-Ferredoxin Fold Fusion Dimer with 4-Glutamate Binding Site and Tryptophan Antenna (Tfd-Ee N6W)


Mono view


Stereo pair view

A full contact list of Terbium with other atoms in the Tb binding site number 1 of Terbium(III)-Bound De Novo Tim Barrel-Ferredoxin Fold Fusion Dimer with 4-Glutamate Binding Site and Tryptophan Antenna (Tfd-Ee N6W) within 5.0Å range: probe atom residue distance (Å) B Occ A:Tb201 b:29.3 occ:1.00 OE2 A:GLU154 2.3 23.0 1.0 OE1 B:GLU154 2.3 24.7 1.0 OE1 B:GLU31 2.4 27.4 1.0 OE2 B:GLU31 2.4 29.7 1.0 OE2 B:GLU154 2.4 27.5 1.0 OE1 A:GLU31 2.4 38.9 1.0 OE2 A:GLU31 2.5 31.3 1.0 OE1 A:GLU154 2.5 24.4 1.0 O B:HOH220 2.7 22.1 1.0 CD B:GLU154 2.7 27.2 1.0 CD B:GLU31 2.7 29.7 1.0 CD A:GLU154 2.7 25.1 1.0 CD A:GLU31 2.8 32.3 1.0 CG B:GLU31 4.2 29.3 1.0 CG B:GLU154 4.2 28.5 1.0 CG A:GLU154 4.3 25.1 1.0 CG A:GLU31 4.3 34.1 1.0 O2 A:EDO202 4.3 31.8 1.0 ND2 A:ASN52 4.3 33.2 1.0 CD1 B:TRP6 4.7 32.0 1.0 O B:HOH217 4.8 44.2 1.0 CD1 A:TRP6 5.0 59.2 1.0

S.J.Caldwell, I.C.Haydon, N.Piperidou, P.S.Huang, M.J.Bick, H.S.Sjostrom, D.Hilvert, D.Baker, C.Zeymer. Tight and Specific Lanthanide Binding in A De Novo Tim Barrel with A Large Internal Cavity Designed By Symmetric Domain Fusion. Proc.Natl.Acad.Sci.Usa 2020.
ISSN: ESSN 1091-6490
PubMed: 33203677
DOI: 10.1073/PNAS.2008535117