Terbium in PDB 8bl6: De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A

The structure of De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A, PDB code: 8bl6 was solved by M.Nadal, J.Roel-Touris, E.Marcos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.59 / 2.80
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	73.841, 73.841, 96.245, 90, 90, 90
R / Rfree (%)	20.2 / 25.7

The binding sites of Terbium atom in the De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A (pdb code 8bl6). This binding sites where shown within 5.0 Angstroms radius around Terbium atom.
In total 3 binding sites of Terbium where determined in the De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A, PDB code: 8bl6:
Jump to Terbium binding site number: 1; 2; 3;

Terbium binding site 1 out of 3 in the De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A


Mono view


Stereo pair view

A full contact list of Terbium with other atoms in the Tb binding site number 1 of De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A within 5.0Å range: probe atom residue distance (Å) B Occ A:Tb202 b:149.1 occ:1.00 OE1 A:GLU10 2.6 120.3 1.0 OE1 A:GLU8 2.6 133.9 1.0 OE2 A:GLU10 2.7 121.6 1.0 CD A:GLU10 2.9 116.4 1.0 NH2 A:ARG144 3.2 149.1 1.0 CD A:GLU8 3.8 140.8 1.0 CG A:GLU8 4.3 131.6 1.0 CZ A:ARG144 4.3 143.2 1.0 CG A:GLU10 4.3 130.7 1.0 NH2 A:ARG17 4.4 142.0 1.0 CB A:GLU8 4.5 104.5 1.0 NE A:ARG144 4.8 127.8 1.0 OE2 A:GLU8 4.8 127.4 1.0 NH1 A:ARG15 4.8 150.5 1.0

Terbium binding site 2 out of 3 in the De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A


Mono view


Stereo pair view

A full contact list of Terbium with other atoms in the Tb binding site number 2 of De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A within 5.0Å range: probe atom residue distance (Å) B Occ A:Tb203 b:197.6 occ:1.00 OE2 A:GLU27 1.9 135.4 1.0 CD A:GLU27 3.1 112.2 1.0 OE1 A:GLU27 3.7 135.4 1.0 CG A:GLU27 4.2 92.2 1.0 CE1 A:HIS29 4.8 98.0 1.0

Terbium binding site 3 out of 3 in the De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A


Mono view


Stereo pair view

A full contact list of Terbium with other atoms in the Tb binding site number 3 of De Novo Single-Chain Immunoglobulin Dimer SCIG12+EF3A within 5.0Å range: probe atom residue distance (Å) B Occ A:Tb204 b:307.5 occ:1.00 OE1 A:GLU67 3.0 114.7 1.0 CE A:LYS83 3.5 191.3 1.0 CD A:LYS83 3.5 183.3 1.0 CD A:GLU67 3.8 126.6 1.0 NZ A:LYS83 4.0 190.7 1.0 CB A:GLU67 4.1 98.1 1.0 CG A:GLU67 4.2 110.3 1.0 OE2 A:GLU67 4.8 139.9 1.0 O A:TRP68 4.9 99.2 1.0 CB A:LYS69 4.9 160.0 1.0 CA A:LYS69 5.0 148.9 1.0

J.Roel-Touris, M.Nadal, E.Marcos. Single-Chain Dimers From De Novo Immunoglobulins As Robust Scaffolds For Multiple Binding Loops. Nat Commun V. 14 5939 2023.
ISSN: ESSN 2041-1723
PubMed: 37741853
DOI: 10.1038/S41467-023-41717-5